Effect and mechanism of intense pulsed laser on skin aging in rats.

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009 May; 34(5): 375-81Yaping X, Wang B, Lu J, Chen J, Zuo C, Tan L, Huang JObjective To explore the effect of intense pulsed laser (IPL) on anti-aging and its mechanism.Methods Rat skin was continuously irradiated ex vivo by IPL at certain wavelengths with different energy densities. The rats were irradiated twice with an interval of 2 weeks. We measured superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, hydroproline (Hyp) content, and the level of apoptosis-related gene B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in the IPL treatment groups, and then compared with those in the model groups and the control group.Results SOD activity and Hyp content in the IPL treatment groups were higher than those in the model groups, and MDA content in the IPL treatment group was lower than that in the model group (P